Noncytotoxic combinations of topical antimicrobial agents for use with cultured skin substitutes
نویسندگان
چکیده
منابع مشابه
Noncytotoxic combinations of topical antimicrobial agents for use with cultured skin substitutes.
Cultured skin grafts are destroyed more easily than split-thickness skin grafts by common burn wound organisms, including gram-negative and gram-positive bacteria and fungi. To increase the survival and engraftment of cultured skin grafts, formulations of antimicrobial agents were tested for cytotoxicity to cultured human keratinocytes and fibroblasts and for activity against common organisms f...
متن کاملFormulation of 'idealized' topical antimicrobial mixtures for use with cultured skin grafts.
In order to develop antimicrobial mixtures which provide broad-spectrum antimicrobial activity for use with cultured cell autografts, several individual antimicrobial agents, in concentrations non-toxic for cells in culture, were tested against a variety of bacteria and Candida spp. isolated from burn patients. An agar well diffusion topical assay was used. Antimicrobials active against Gram-po...
متن کاملCytotoxicity Testing of Topical Antimicrobial Agents on Human Keratinocvtes and Fibroblasts for Cultured Skin
Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited because of destruction by microbial contamination. To evaluate candidate antimicrobial agents for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibrobla...
متن کاملSelection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity.
Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited due to destruction by microbial contamination. To evaluate candidate drugs for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibroblasts, and to common ...
متن کاملCultured skin substitutes: a review.
Skin substitutes composed of cultured cells and biopolymers provide alternative materials for study of skin biology and pathology, treatment of skin wounds, safety testing of consumer products, and therapeutic delivery of gene products. Most frequently, substitutes for epidermis consist of cultured keratinocytes and dermal substitutes consist of resorbable biopolymers populated with cultured fi...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1995
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.39.6.1324